METHOD FOR PRESERVING ENZYME

摘要

The problem is to provide a method for inexpensively and simply preserving an enzyme of a microbial biomass obtained by culturing and improve the enzyme activity during preservation. Provided is a method for preserving nitrile hydratase, characterized in that microbes having nitrile hydratase activity are cultured while being protected from light, and the resulting biomass is crushed using a high-pressure homogenizer and preserved while being protected from light.

主权项

1. A method for preserving nitrile hydratase, said method comprising:
(1) culturing a microorganism having a nitrile hydratase activity while protecting them from light, (2) disrupting the cultured microbial cells from the microorganism using a high-pressure type homogenizer, and (3) preserving the disrupted microbial cells for 4 to 24 hours at 4 to 37° C.: while protecting them from light.