METHODS AND ARTICLES FOR DETECTING DEOXYRIBONUCLEASE ACTIVITY

摘要

The disclosure provides articles and methods useful for detecting a discrete source of DNase activity. DNase-producing microorganisms can be detected. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating DNase-producing microorganisms.

主权项

1. A method of detecting deoxyribonuclease activity, comprising:
providing
a hydrogel comprising a DNase indicator system including methyl green and DNA, wherein the concentration of methyl green in the hydrogel is at least about 5 μg/mL to about 20 μg/mL;a sample suspected of containing a discrete source of deoxyribonuclease activity; andan imaging system; contacting, for a predetermined period of time, the sample and the hydrogel; obtaining an image of the hydrogel with the imaging system; and detecting a discrete source of deoxyribonuclease activity, wherein detecting deoxyribonuclease activity comprises displaying, printing, or analyzing the image of the hydrogel.