PURIFICATION

摘要

The invention provides methods of purifying one or more biological analytes from a sample. The method comprises incubating the sample with nanoparticles and centrifuging a biphasic system. The biological analytes can be one or more of an enzyme, a protein, a nucleic acid, an organelle, a cell, a bacteria, a virus, or any other biological material which requires purification.

主权项

1. A method of purifying a biological analyte from a sample comprising;
incubating a sample comprising a biological analyte with nanoparticles; adding an organic phase or an aqueous phase as necessary to produce a biphasic mixture; centrifuging the mixture until a sedimented aqueous droplet comprising the nanoparticle bound biological analyte is formed;wherein the organic phase is immiscible with water and has a density greater than greater than 1.0 g/cm3.