CELL LINE ADAPTED TO PROTEIN-FREE AND LIPID-FREE MEDIUM, METHOD FOR PRODUCING THE CELL LINE, AND MEDIUM FOR THE CELL LINE

摘要

PROBLEM TO BE SOLVED: To provide a Chinese Hamster Ovary (CHO)-derived cell line which is free of safety concerns, can be stably used for production of recombinant proteins, can proliferate in a suspended state, and can be cultured at low costs; and to provide a method for adapting CHO cells by using a protein-free and lipid-free medium, a medium to be used for the method, and so on.SOLUTION: This invention relates to a cell of a cell line derived from Chinese Hamster Ovary (CHO) cells, where the cell line is adapted to a protein-free and lipid-free medium. The cell can proliferate in a suspended state in a protein-free and lipid-free medium comprising no exogenous growth factors.