发明名称 |
GENETICALLY ENCODED FRET-BASED MMP-9 ACTIVITY BIOSENSOR AND USE THEREOF |
摘要 |
The present invention relates to a genetically encoded FRET-based biosensor to monitor the activity of matrix metalloproteinase 9 (MMP-9). MMP-9 is an extracellular acting endopeptidase implicated in both physiological and pathological processes. A genetically encoded FRET biosensor anchored in the cellular membrane allows studying the proteolytic activity of MMP-9 with high spatiotemporal resolution at the exact region of MMP-9 action on the cell. Applicability of the biosensor, both in vitro and in vivo in living cells, has been demonstrated by ratiometric analysis of cleavage of the biosensor by a purified auto-activating mutant of MMP-9. |
申请公布号 |
WO2015022646(A2) |
申请公布日期 |
2015.02.19 |
申请号 |
WO2014IB63883 |
申请日期 |
2014.08.12 |
申请人 |
INSTYTUT BIOLOGII DOSWIADCZALNEJ IM. MARCELEGO NENCKIEGO POLSKA AKADEMIA NAUK |
发明人 |
STAWARSKI, MICHAL;WLODARCZYK, JAKUB;KACZMAREK, LESZEK |
分类号 |
G01N33/542 |
主分类号 |
G01N33/542 |
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