Method for the determination of polysorbate

摘要

The present invention relates to a method for the determination of polysorbate in a protein-containing sample. The method of the present invention involves the pretreatment of the sample by alkaline hydrolysis followed by colorimetric determination on the basis of the metal complex of the analyte with a thiocyanate reagent, the complex being extracted in an immiscible organic solvent. The alkaline hydrolysis accounts for the removal of the interfering proteins and enhance selectivity over surfactants similar to the analyte, e.g. for Tween 80 over Triton X-100.