Formalin-Free Fixation Agent For Histological Stains of Tissue Samples

摘要

A fixation agent including at least one polyamine and at least one acidification agent. The quality and staining of tissue samples or tissue sections that have not been fixed with formalin is improved, with higher brilliance and color stability of the stain, allowing the tissue to be cut with a better quality without artifacts.

主权项

1. A fixation agent for tissue samples, comprising:
at least one polyamine, selected from the group consisting of urotropine, dimethylol dihydroxyethylene urea, mono-, di-, tri-, tetra-, penta- or hexamethylol melamine, tetramethylol acetylene diurea, dimethylol propylene urea, acetoguanamine or 5,5-dimethylhydantoin, and mixtures thereof, and at least one acidification agent selected from the group of free monovalent, bivalent or polyvalent acid consisting of oxalic acid, fumaric acid, tartaric acid, maleic acid, succinic acid, hydrochloric acid, acetic acid, propionic acid, formic acid, mono-, di- or tri chloroacetic acid or chloroacetic acid, boric acid, phosphoric acid, and mixtures thereof,wherein the polyamine reacts with protons released from the acidification agent, thus forming or releasing aldehydes, and the molar ratio between the molar concentration of releasable aldehyde per mol of amine to the molar concentration of releasable protons per mol of acid lies in a range from 1:0.7 to 1:1.5.