Measuring Method of Enzyme Activity

摘要

Providing is a new enzyme assay method for enzymes having a water-insoluble or substantially water-insoluble substrate. In the method for measuring enzymatic activity, a prescribed amount of an enzyme is disposed on a part of the surface of a gel comprising dispersoids, at least some of which are the substrate of the enzyme. Recesses formed in the surface of the gel by the action of the enzyme are measured, and the enzyme activity is calculated on the basis of the measurement results and the amount of the enzyme. The measurement of the recesses formed in the surface of the gel is performed using a method for measuring the shape and the volume of the recesses, a method for measuring changes in the optical transmittance of the gel due to the formation of the recesses, or a method for measuring changes in the optical reflectance of the gel surface due to the formation of the recesses.

主权项

1. A method of measurement of activity of an enzyme comprising:
positioning a prescribed quantity of an enzyme on a portion of the surface of a gel body at least part of the dispersoid of which is comprised of the substrate of the enzyme; measuring the pits formed on the surface of the gel body by the action of the enzyme; and calculating the activity of the enzyme based on the measurement results and the prescribed quantity.