| 摘要 |
The present invention refers to an aqueous solution for use as medium for the specific binding reaction of a binding pair, wherein a first binding member recognizes its complementary second binding member. The solution contains a) a buffer to control pH; b) a compound A selected from a compound defined by the general formula I R1-[[CR2R3]p-O]q-R4, wherein R1 is hydrogen or hydroxy group, R2 for each unit independently is hydrogen or hydroxy group, R3 is hydrogen, methyl group, or ethyl group, R4 is hydrogen or alkyl group, p is an integer of from 2 to 10 and q is an integer of from 1 to 100, with the proviso that the compound at least carries two hydroxy groups; a polyol; or saccharide; and c) a non-ionic detergent. |
| 主权项 |
1. A method for identifying the presence of an antigen or an antibody in a sample, the method comprising:
contacting the sample with a complementary capture antibody or a capture antigen in an aqueous solution, specifically binding the antigen or the antibody to the complementary capture antibody or capture antigen, respectively; and detecting the presence of the antigen or antibody bound to the complementary capture antibody or capture antigen with a detector antibody that binds to either (a) the antigen, which is bound to the complementary capture antibody, or (b) the antibody, which is bound to the capture antigen,said sample further comprising a sample component comprising an interfering protein other than an antibody or a triglyceride, wherein said sample component interferes with the specific binding between the antigen or the antibody in the sample and the complementary capture antibody or capture antigen or detector antibody;said aqueous solution comprising:a) a buffer to control pH;b) a compound A selected from the group consisting of: a compound defined by the general formula I R1-[[CR2R3]p-O]q-R4, wherein R1 is hydrogen or hydroxy group, R2 for each unit independently is hydrogen or hydroxy group, R3 is hydrogen, methyl group, ethyl group, R4 is hydrogen or alkyl group, p is an integer of from 2 to 10 and q is an integer of from 1 to 100, with the proviso that the compound at least carries two hydroxy groups; a polyol; and a saccharide; andc) a non-ionic detergent;andwherein said aqueous solution reduces the binding of said interfering sample component in the sample to any one or more of the following: complementary capture antibody or capture antigen or detector antibody or the antigen or the antibody of the sample to be detected, thereby reducing interference by the interfering sample component with the specific binding of said antigen or antibody to the complementary capture antibody or capture antigen or detector antibody, compared to conducting said specific binding in the absence of said aqueous solution. |
