SOLUBLE ENGINEERED MONOMERIC FC

摘要

Fc domains and CH3 domains are disclosed that bind the neonatal Fc (FcRn) receptor and are at least 99% monomeric. Monomeric Fc domain molecules and CH3 domain molecules are disclosed herein that include a monomeric Fc domain or a monomeric CH3 domain and an effector molecule. In some embodiments, the monomeric Fc or monomeric CH3 domain include amino acid substitutions and/or CDR insertions in the beta strands such that they specifically bind an antigen. Methods for using these monomeric Fc domains, monomeric CH3 domains, monomeric Fc domain molecules and CH3 domain molecules are also provided.

主权项

1. An isolated monomeric Fc polypeptide comprising a CH2 and a CH3 domain, wherein the Fc polypeptide comprises
(a) the amino acid sequence set forth as SEQ ID NO: 1, wherein X1 is Y, K or S, wherein X2 is Y, S or R, wherein X3 is A, L or H, wherein X4 is R, V or K, wherein X5 is F, R or E; wherein X6 is Y, M, A, or K; and wherein X7 is K, A or Y, or (b) the amino acid sequence set forth as SEQ ID NO: 44, wherein X1 is Y, K or S, wherein X2 is Y, S or R, wherein X3 is A, L or H, wherein X4 is R, V or K, wherein X5 is F, R or E; wherein X6 is Y, M, A, or K; and wherein X7 is K, A, wherein the Fc domain is monomeric and binds the neonatal Fc receptor.