| 摘要 |
<p>A serum-free culture medium capable of supporting in vitro culture of mammalian embryos on a substrate from a pre-implantation stage of development to a post-implantation stage of development comprises an insulin receptor agonist (e.g. insulin, IGF-I or IGF-II), an oestrogen receptor agonist (e.g. beta-estradiol) and a progesterone receptor agonist (e.g. progesterone). The medium may also comprise transferrin, sodium selenite, ethanolamine, L-glutamine, sodium pyruvate, amino acids, reducing agents and/or agonists of the activin type 1 or type 2 receptors. The insulin receptor agonist, oestrogen receptor agonist and progesterone receptor agonist may also be provided as a culture medium supplement. Methods of culturing mammalian embryos, and methods of testing the effects of agents on embryo development, are also provided. The medium may also be used for the culture of stem cells, such as embryonic stem cells.</p> |
