Vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same

摘要

Disclosed herein is an inducible high-expression cassette comprising a dihydrofolate reductase (DHFR) promoter from which GC-rich repeat sequences are partially or entirely removed, the cassette capable of more effectively improving a gene amplification system. Also disclosed are an expression vector comprising the inducible expression cassette and optionally a gene encoding a recombinant protein of interest, an animal cell line transformed with the expression vector, and a method of mass producing and purifying a recombinant protein by culturing the transformant. The present invention enables the shortening of the time required to establish a cell line producing a recombinant protein of interest at high levels using a low concentration of a DHFR inhibitor, thereby allowing more effective production of the recombinant protein.

主权项

1. An isolated inducible high-expression cassette comprising the nucleotide sequence of SEQ ID NO. 10, wherein one or more CCGCCC repeat sequences are removed from the GC-rich region of positions 8 to 67 of SEQ ID NO. 10.