<i>Paenibacillus </i>sp. HPL-3 strain producing xylanase having heat-resistance, a wide range of optimum pH and high activity, a novel xylanase separated from the strain, and a method for mass-production of the same using the transformant originated from the strain

摘要

The present invention relates to the novel Paenibacillus sp. strain, and the novel protein isolated from the same. More particularly, the present invention relates to the novel Paenibacillus sp. strain producing xylanase, and the novel xylanase having high activity at high temperature and in a wide range of pH, and a production method of the same. The Paenibacillus sp. HPL-3 strain (KCTC11987BP) and the xylanase of the present invention demonstrates high activity at high temperature or in a wide range of pH to decompose xylan, the major component of various lignocellulosic biomass, so that they can be effectively used for the production or development of bio-fuel, alternative material, performance chemical, bio-polymer, food and feeds, etc.

主权项

1. A Xylanase having
a) the amino acid sequence set forth as SEQ. ID. NO: 4; b) the amino acid sequence having at least 90% homology with the amino acid sequence set forth as SEQ. ID. NO: 4; c) the amino acid sequence encoded by the nucleotide sequence set forth as SEQ. ID. NO: 3; or d) the amino acid sequence encoded by DNA hybridized with the DNA containing the nucleotide sequence set forth as SEQ. ID. NO: 3 in the strict condition comprising at least two washings in 0.1×SSC, 0.1% SDS at 65° C., which is identical in its function to the protein containing the amino acid sequence set forth as SEQ. ID. NO: 4; wherein the xylanase has the characteristics (i)-(iii); (i) molecular weight: approximately 65 KDa on SDS-PAGE; (ii) maximum activity in the range pH 5-pH 11; and (iii) maximum activity at the temperature of 50-60° C.