Identification of microRNAs (miRNAs) in fecal samples as biomarkers for gastroenterological cancers

摘要

A simple, rapid, inexpensive, and promising commercial biomarker assay method for multiple diseases is described herein. The present invention detects miRNA-based biomarkers in human stool specimens. The method of the present invention amplifies miRNA directly from stool specimens without any prior miRNA extraction. Differential expression of specific microRNAs in stool of colorectal cancer CRC and adenoma patients suggest fecal microRNAs as a novel potential biomarker for colorectal neoplasia detection. The method of the present invention has diagnostic, prognostic, and therapeutic relevance for gastroenterological cancers/colorectal cancer and as well as further acquired or hereditary GI diseases.

主权项

1. A method of processing a biological sample comprising ribonucleic acids of a subject for characterization of one or more target MicroRNAs (miRNAs) in the sample comprising the steps of:
a) mixing the sample with RNase free water, a salt solution, or both to form a suspension; b) centrifuging the suspension; c) separating an miRNA-containing supernatant from the centrifuged suspension; d) amplifying the one or more miRNAs without prior RNA extraction; wherein the sample containing ribonucleic acid is mixed with RNase free water, a salt solution, or both without a prior extraction.