摘要 |
<p>The present invention relates to a method for detecting somatic mutation using a next generation sequencing technique. More specifically, target nucleic acid is amplified under the presence of a PNA probe complementarily coupled with the target nucleic acid, and the occurrence or concentration of somatic mutation is determined by performing next generation sequencing on an amplified byproduct. The method of the present invention can detect a mutation of 0.01% or less, so that mutated genes mixed in normal genes can be effectively detected.</p> |