| 摘要 |
<p>1,230,065. Nucleic acid. MERCK & CO. Inc. 22 Sept., 1969 [27 Sept., 1968], No. 46576/69. Heading C3H. A method of isolating double-stranded ribonucleic acid (DS-RNA) from a fermentation broth of Escherichia coli cells which have been infected with MS2 coliphage or the MU9 mutant of this coliphage includes the step of lysing the cells to release the DS-RNA, adding a proteolytic enzyme which does not degrade nucleic acid, incubating the mixture to degrade the protein, adding phenol to extract the degraded protein, and centrifuging and recovering the aqueous phase containing the DS-RNA. The DS-RNA may be precipitated from the aqueous phase by cetyl trimethylammonium bromide, dissolved in a buffer and further purified by chromatography on hydroxylapatite.</p> |
