Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

摘要

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

主权项

1. A nucleic acid construct or recombinant expression vector comprising an isolated polynucleotide comprising a nucleotide sequence that encodes a GH61 polypeptide having cellulolytic enhancing activity, wherein the isolated polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from:
(a) a GH61 polypeptide having at least 95% sequence identity to amino acids 24 to 436 of SEQ ID NO: 6; (b) a GH61 polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) nucleotides 70 to 1483 of SEQ ID NO: 5, (ii) the cDNA sequence of nucleotides 70 to 1483 of SEQ ID NO: 5, or (iii) the full-length complement of (i) or (ii), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.; (c) a GH61 polypeptide encoded by a polynucleotide having at least 99% sequence identity to nucleotides 70 to 1483 of SEQ ID NO: 5, or a GH61 polypeptide encoded by the cDNA of nucleotides 70 to 1483 of SEQ ID NO: 5; and (d) an isolated fragment of a GH61 polypeptide of (a), (b), or (c) that has cellulolytic enhancing activity.