METHOD FOR IDENTIFICATION OF THE SEQUENCE OF POLY(A)+RNA THAT PHYSICALLY INTERACTS WITH PROTEIN

摘要

The invention relates to an in vitro method for identifying the sequence of one or more poly(A)+RNA molecules that physically interacts with protein. The present invention provides a method to define the protein-bound transcriptome under any given cellular condition, such as a disease condition or after treatment with any given substance, drug, or other cellular perturbation. The invention also relates to a method for identification of a drug target and a method for the identification of one or more biomarkers, preferably for identification of a panel of biomarkers, for any given medical condition, comprising the method of the invention.

主权项

1. In vitro method for identifying the sequence of one or more poly(A)+RNA molecules that physically interacts with protein, comprising:
a) formation of poly(A)+RNA-protein complexes via cross-linking, b) isolation of poly(A)+RNA-protein complexes by
binding of poly(A)+RNA-protein complexes with poly(A)+RNA-binding oligonucleotides, preferably oligo(dT) oligonucleotides, andremoval of unbound poly(A)+RNA, followed by c) removal of total protein, and d) identification of poly(A)+RNA sequences.