| 摘要 |
Hybrid enrichment technology as applied to enhancing agricultural production, for example including, but not limited to, crop production, pesticide development, crop trait development, and pest control. The methodology pools samples prior to library production, thus reducing cost and increasing efficiency of sequencing across- and within-species targets. |
| 主权项 |
1. A method of generating cross-species genetic profiles using solution-based sequence capture, target enrichment probes to reconstruct a phylogenetic tree at a range of phylogenetic timescales in order to develop a pesticide, comprising the steps of:
sequencing a first genetic fragment acquired from a first species selected from a broader group, said first genetic fragment including a first conserved region and a first non-conserved region, said first conserved region coding for a first allele; sequencing a second genetic fragment acquired from a second species selected from said broader group, said second genetic fragment including a second conserved region and a second non-conserved region, said second conserved region coding for a second allele homologous to said first allele, said first species and said second species selected from said broader group; establishing a conservation standard and a uniqueness standard, said first conserved region and said second conserved region meeting said conservation standard and said uniqueness standard; designing said solution-based sequence capture, target enrichment probes from said first genetic fragment and said second genetic fragment, such that said first conserved region and said second conserved region of said probes are respectively flanked by said first non-conserved region and said second non-conserved region, said first non-conserved region disposed adjacent to said first conserved region and said second non-conserved region disposed adjacent to said second conserved region; preparing a library containing said target enrichment probes; applying said library to a targeted locus; sequencing said targeted locus as a result of a successful capture of said targeted locus, whereby sequence capture using said first and second conserved regions permits capture of large numbers of loci model species and highly-divergent non-model species, generation of said loci useful at said range of phylogenetic timescales, and production of said loci capable of resolving said phylogenetic tree despite gene tree discordance; and developing said pesticide by targeting sequences resulting from said sequencing of said targeted locus. |
