发明名称 |
METHOD |
摘要 |
The present invention provides a method of quantifying the activity of a protein modifying enzyme in a sample, comprising: (i) grouping modified peptides from a first sample and modified peptides from a second sample into a single group according to one of the following parameters: (a) modified peptides having a modification site that is modified by the same protein modifying enzyme; or (b) modified peptides having a modification site that is part of the same modification motif; (ii) calculating enrichment of the modified peptides from the first sample compared to the modified peptides from the second sample in the group; and (iii) calculating the statistical significance of said enrichment; wherein a statistically significant enrichment is indicative of a protein modifying enzyme being activated in the first sample compared to the second sample. In some embodiments, the method further comprises identifying modified peptides in a first sample and a second sample using mass spectrometry (MS) prior to step (i). |
申请公布号 |
US2015044713(A1) |
申请公布日期 |
2015.02.12 |
申请号 |
US201314384151 |
申请日期 |
2013.03.08 |
申请人 |
QUEEN MARY UNIVERSITY OF LONDON |
发明人 |
Rodriguez Cutillas Pedro |
分类号 |
C12Q1/48 |
主分类号 |
C12Q1/48 |
代理机构 |
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代理人 |
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主权项 |
1. A method of antifying the activity of a protein modifying enzyme in a. sample, comprising:
(i) grouping modified peptides from a first sample and modified peptides from a second sample into a single group according to one of the following parameters:
(a) modified peptides having a modification site that is modified by the same protein modifying enzyme; or(b) modified peptides having a modification site that is part of the same modification motif; (ii) calculating enrichment of the modified peptides from the first sample compared to the modified peptides from the second sample in the group; and (iii) calculating the statistical significance of said enrichment; wherein a statistically significant enrichment is indicative of a protein modifying enzyme being activated in the first sample compared to the second sample. |
地址 |
London GB |