METHOD FOR DETERMINING THE ABILITY OF AN ANTIBODY TO KEEP CELLS CLOSE TO ONE ANOTHER

摘要

The invention relates to a method for determining the ability of a candidate antibody to keep a first cell and a second cell close to one another, said method comprising the following steps:
bringing said candidate antibody in contact with (i) a first cell expressing, in the extracellular portion of its plasma membrane, a first protein that is known to be or is suspected of being recognized by the candidate antibody and (ii) a second cell expressing, in the extracellular portion of the plasma membrane, a second protein that is also known to be or suspected of being recognized by the candidate antibody, each of the proteins being labeled with a member of a pair of FRET partners, andmeasurement of the FRET signal and comparison with the signal measured in the absence of the candidate antibody.;The invention also relates to a reagent kit for carrying out this method.

主权项

1. A method for determining the ability of a candidate antibody to keep a first cell and a second cell close to one another, this method comprising the following steps:
bringing the following elements in contact:
(i) a first cell expressing, in the extracellular portion of its plasma membrane, a first protein that is known to be or is suspected of being recognized by the candidate antibody, said first protein being labeled directly or indirectly with the first member of a pair of FRET partners,(ii) a second cell expressing, in the extracellular portion of the plasma membrane, a second protein that is also known to be or suspected of being recognized by the candidate antibody, said second protein being labeled directly or indirectly with the second member of said pair of FRET partners,(iii) said candidate antibody; measuring of the FRET signal and comparison with the signal measured in the absence of the candidate antibody, wherein an increase in the signal measured in the presence of the candidate antibody relative to that measured in its absence is indicative of the ability of said antibody to keep the first cell and the second cell close to one another.