摘要 |
PROBLEM TO BE SOLVED: To provide a cell sheet-production method using a cell culture medium comprising no impurity component from a production process, which is an obstacle to clinic application.SOLUTION: Provided are a cell sheet-production method comprising cultivation of cells having a density allowing formation of a cell sheet without substantial proliferation, in a cell culture medium not comprising an effective amount of growth factor; a cell sheet produced by the same method; and a culture medium used in the same method. |