METHOD FOR THE DETECTION OF BIOLOGICAL MOLECULES USING A TWO PARTICLE COMPLEX

摘要

Methods, compositions and kits for detecting analytes of interest in a sample using electrogenerated chemiluminescence are provided. Compositions comprising at least one solid support that entraps or contains an electrogenerated chemiluminescent moiety also provided.

主权项

1. A method of detecting an analyte of interest in a sample, comprising
(a) forming a composition comprising:
(A)k (B)u, (C), (D)x wherein A is an electrogenerated chemiluminescent (ECL) moiety which can be induced to repeatedly emit electromagnetic radiation by direct exposure to an electrochemical energy source; and the ECL moiety is soluble in organic solvent and insoluble in aqueous solvent; B is a synthetic organic polymer bead comprising a plurality of the ECL moieties entrapped within its interior; and B is either linked to the analyte of interest or linked to a first specific binding partner of the analyte of interest; C is the sample which may contain the analyte of interest; and D is a magnetizable bead which is either linked to the analyte of interest or linked to a second specific binding partner of the analyte of interest; wherein k, u, and x are each an integer equal to or greater than 1; (b) separating a complex comprising A, B, D and the analyte of interest from other components of the composition; (c) inducing the ECL moiety in the complex to repeatedly emit electromagnetic radiation by directly exposing the moiety to electrochemical energy; and (d) detecting the emitted electromagnetic radiation and thereby detecting the presence of the analyte of interest, provided that B and D are not both linked to the analyte of interest.