HIGH-THROUGHPUT PLATFORM FOR IN-VIVO SUB-CELLULAR SCREENS ON VERTEBRATE LARVAE

摘要

High throughput system for in vivo screens on vertebrate larvae. The system includes a source of vertebrate larvae in a liquid medium and loading tube means for aspirating a larva. A detector assembly is provided to differentiate passage of a larva from bubbles and/or debris. An imaging means is provided for both confocal imaging and wide-field fluorescence imaging of the larva. A laser is provided for optical manipulation of the larva.

主权项

1. A high-throughput system for in vivo screens comprising:
a source of a specimen organism in a liquid medium; a pump configured to load the specimen organism in the liquid medium into a transparent tube; an automatic optical means to position the specimen organism in the transparent tube within a field-of-view of an imaging apparatus for microscopy through the transparent tube; and a motor and capillary assembly operable to rotate the transparent tube, thereby rotating the specimen organism within the field-of-view of the imaging apparatus.