Recombinant butyrylcholinesterases and truncates thereof

摘要

Isolated nucleic acids encoding polypeptides that exhibit butyrylcholinesterase (BChE) enzyme activity are disclosed, along with molecular criteria for preparing such nucleic acids, including codon optimization. Methods of preparing modified and/or truncated BChE molecules having selected properties, especially selective formation of monomers, are also described. Vectors and cells containing and/or expressing the nucleic acids are also disclosed.

主权项

1. An isolated nucleic acid that encodes a polypeptide having butyrylcholinesterase—(BChE) enzyme activity, wherein said nucleic acid has at least 90% identity to the nucleotide sequence of SEQ ID NO: 1, or the complement of said isolated nucleic acid.