| 发明名称 | Method for producing high-purity soluble thrombomodulin | ||
| 摘要 | A method is provided for producing soluble thrombomodulin substantially not containing a denatured product of the soluble thrombomodulin that is generated under acidic conditions, which comprises: subjecting the soluble thrombomodulin-containing material to an anion exchanger or hydroxyapatite; and carrying out linear gradient elution, stepwise gradient elution, or gradient elution in which linear gradient elution is combined with stepwise gradient elution under separation conditions in which the denatured product of the soluble thrombomodulin can be separated, wherein the gradient is a salt concentration gradient, so as to obtain an elution fraction containing soluble thrombomodulin that does not substantially contain the denatured product of the soluble thrombomodulin, either (a) after the position of a fraction has previously been confirmed, or (b) while confirming the elution fraction. | ||
| 申请公布号 | US8952137(B2) | 申请公布日期 | 2015.02.10 |
| 申请号 | US201213557645 | 申请日期 | 2012.07.25 |
| 申请人 | Asahi Kasei Pharma Corporation | 发明人 | Ohigashi Susumu |
| 分类号 | A23J1/00;C07K1/18;C07K14/745;C07K1/16 | 主分类号 | A23J1/00 |
| 代理机构 | Birch, Stewart, Kolasch & Birch, LLP | 代理人 | Birch, Stewart, Kolasch & Birch, LLP |
| 主权项 | 1. A method for producing soluble thrombomodulin substantially not containing a denatured product of the soluble thrombomodulin that may be generated from the soluble thrombomodulin under acidic conditions, which comprises: (1) a step of leaving the soluble thrombomodulin under acidic conditions of pH 5 or less; (2) a step of subjecting a soluble thrombomodulin-containing material that contains or is suspected to contain a denatured product of the soluble thrombomodulin, which is obtained by step (1), to an anion exchanger or hydroxyapatite; and (3) a step of obtaining a soluble thrombomodulin-containing fraction that does not substantially contain a denatured product of the soluble thrombomodulin under separation conditions in which the soluble thrombomodulin can be separated from a denatured product of the soluble thrombomodulin, wherein the anion exchanger comprises a buffer solution of pH 5 to 9, and wherein the hydroxyapatite comprises a buffer solution of pH 6 to 9, wherein the content of the denatured product of the soluble thrombomodulin in the soluble thrombomodulin that does not substantially contain the denatured product of soluble thrombomodulin is 3% or less. | ||
| 地址 | Tokyo JP | ||