| 主权项 |
1. An apparatus for cell separation, the apparatus comprising:
a channel having an inlet and an outlet, the inlet configured to receive an initial carrier fluid containing at least a first cell; a flow surface extending between the inlet and the outlet of the channel, wherein the flow surface includes a patterned surface that includes one or more indentations fabricated to exhibit at least one characteristic of the first cell, wherein the patterned surface is configured to selectively capture at least the first cell and to permit the carrier fluid to flow therethrough, and wherein the patterned surface of the flow surface is at least partially coated with one or more photo-responsive azobenzene compound molecules having a first region configured to bind to a surface of the flow surface and having a second photo-responsive region configured to change conformation between a first more hydrophobic conformation and a second more hydrophilic conformation responsive to irradiation from an optical energy source,
wherein the first more hydrophobic conformation displays a diazo functional group (N═N) on a first exposed surface of the one or more photo-responsive azobenzene compound molecules allowing the at least one indentation to receive and interact with the first cell, andwherein the second more hydrophilic conformation displays a polar group on a second exposed surface of the one or more photo-responsive azobenzene compound molecules permitting release of the first cell from the at least one indentation; anda detector positioned downstream from and in fluid communication with the outlet of the channel, the detector configured to detect flow of one or more target cells flowing from the outlet of the channel. |
