Enhanced immunoassay sensor

摘要

Disclosed herein are devices for detecting the presence of a target analyte in a fluid sample. The biosensor device can comprise at least a reaction chamber and a detection chamber. The device can comprise a amplifying mechanism such that one target analyte molecule present in the fluid sample can lead to generation/activation of multiple detection agent molecules, and therefore, an amplified signal. Also disclosed are the methods of manufacturing and using such a biosensor device.

主权项

1. A device for detecting a target analyte in a fluid sample, the device comprising:
a reaction chamber, wherein the reaction chamber comprises internal surfaces, a binding agent and a probe agent, the probe agent comprising a binding partner and a vehicle, wherein the binding partner is bound to the vehicle, wherein the target analyte in the fluid sample can react with the binding agent or the binding partner, wherein the vehicle comprises a plurality of copies of an activating agent; a detection chamber, wherein the detection chamber comprises a detecting agent, wherein the activating agent can activate the detecting agent; and a fluid passageway between the reaction chamber and the detection chamber, wherein the device is adapted to move the reacted fluid sample from the reaction chamber to the detection chamber through the fluid passageway via capillary action, and wherein the presence of the target analyte in the fluid sample at a concentration results in a change in the amount of probe agent that moves with the reacted fluid sample to the detection chamber, wherein the change is detectable in the detection chamber and dependent on at least a threshold of the concentration.