发明名称 Method and device for purifying nucleic acids
摘要 The invention concerns a method for isolating and purifying nucleic acids from large sample volumes and a device suitable for this. The device comprises a funnel, a separating column, and a tab which are detachable from each other. The method according to the invention in which the device according to the invention is used ensures a higher yield of nucleic acids.
申请公布号 US8951780(B2) 申请公布日期 2015.02.10
申请号 US200812261476 申请日期 2008.10.30
申请人 Roche Molecular Systems, Inc. 发明人 Ruhstorfer Maximilian;Buchberger Bernd;Donner Horst;Rainer Alois
分类号 C12M3/00;B01L3/00;B01D29/05 主分类号 C12M3/00
代理机构 代理人 Chang David J
主权项 1. A device for purifying or isolating nucleic acids comprising: a funnel, the funnel comprising an upper part comprising an annular flange which projects outwards and surrounds an inlet opening at its upper end, the flange for holding the device in a vessel, the upper part tapering toward a shaft having an outlet opening and a holder having two opposing grooves at its lower end, a separating column the separating column comprising an inlet opening at its upper end, an outlet opening at its lower end, and an annular flange at its upper end for holding the column flush and tight against the lower end of the funnel whereby the outlet of the funnel and the inlet of the separating column are functionally connected, wherein the annular flange of the separating column is held by the holder, and wherein a nucleic acid binding material is located in front of the outlet opening of the separating column, and a tab consisting of a small plate with two opposing arms that can sit in the holder extending in the holder grooves oriented at a cross-section to a longitudinal axis of the device and having a side opening such that the tab can be removed by pulling the tab along an axis of the grooves of the holder, wherein the tab has an inner edge extending under and supporting the annular flange of the separating column, wherein the separating column is secured against sliding off the funnel shaft by the tab, and wherein the funnel, separating column, and tab are detachable from each other.
地址 Pleasanton CA US