Markers for transformed epithelium and potential targets for therapy of cancer of the gingivo buccal complex

摘要

Systematic comparisons of samples of adjacent clinically non malignant and tumor tissue from cancer of the gingivo buccal complex obtained during surgical resection of the tumor revealed significant differences in protein expression between the cancer tissue and the adjacent clinically non malignant tissue in each patient. This study has identified a set of new proteins which can differentiate between the epithelial tissue from cancer patients which may be useful for high-through put early detection, prognosis, and potential targets for therapy.

主权项

1. A method for identifying co-expressed differentiator marker proteins comprising:
a. providing a first sample from a cancer patient comprising malignant tissue from the gingivo-buccal area of the oral cavity of said cancer patient; b. providing a second sample comprising non malignant tissue from the gingivo-buccal area of the oral cavity of said cancer patient; c. micro-dissecting transformed epithelial cells from frozen sections of the malignant tissue and the non transformed epithelial layer from frozen sections of the non malignant tissue; d. separating proteins of lysates prepared from the micro-dissected transformed and non transformed epithelial cells from (c) by two dimensional gel electrophoresis and staining with silver; e. generating micro-dissected epithelial cell protein profiles from the samples in c) and d); f. comparing the micro-dissected epithelial cell protein profiles generated in step (e) and generating a short list of protein spots commonly expressed in both normal and transformed epithelium; g. punching out the short listed protein spots from a silver stained two dimensional gel of micro-dissected transformed epithelial cells; h. destaining and drying the protein spots and digesting obtained protein with trypsin; i. analyzing eluted dried peptides by mass spectroscopy to obtain identities of proteins; j. comparing the expression of each short-listed protein spot in non malignant and transformed epithelial cells to identity a set of co expressed differentiator marker proteins for the transformed epithelium from the group consisting of lactate dehydrogenase, alpha enolase, prohibitin, cathepsin D, apolipoprotein A-I, tumor protein translationally controlled-1, a stratifin (SFN) family protein, 14-3-3σ, tropomyosin and 14-3-3ζ (YWHAZ); and k. identifying key differentiator protein spots using Linear Discriminator Analysis.