| 摘要 |
FIELD: biotechnology.SUBSTANCE: method for culturing calanoid copepods Calanus euxinus (Black Sea calanus) refers to marine aquaculture and can be used for performing experiments in marine biology, physiology and biochemistry, and for biological tests in marine toxicology, as well as in useful larval sea fish growing. According to the method, calanus females caught from the natural environment are kept in a disinfectant solution at temperate 15°C for 1.5 hours with microalgae Exuviaella cordata added; that is followed by performing a preparation procedure for synchronising mass production of eggs used to produce synchronous hatching of nauplii and synchronous birth cohorts of calanus. The advantages of the method consist in the fact that optimal thermal, throphic and density environment are presented for the first time to synchronise and standardise the processes of egg production by calanus females, calanus egg development and hatching, young calanus development and growth until reaching the last life stages. The egg disinfection enables eliminating the pathogenic microorganisms affecting both calanus's ability to survive, and larval fish's ability of survive when using them as living feed.EFFECT: method enables performing the pre-assessment of the quantity and quality of the produced material. |
