Preliophic molecular using electric fields and gradients for manipulating molecules

摘要

Methods of inducing movement and interaction of molecules in an electric field of an electrophoretic device that is configured to cause molecules to move on a convergent path to mimic a living cell. The electrophoretic device is constructed to support an isoelectric focusing medium, such as a gel on a gel plate with spaced charged poles having different configurations to induce non-parallel migration, and in particular to induce convergent migration wherein spacially separated groups of like-kind molecules are driven on pathways to proximity with other group or groups of like-kind molecules. In this manner researchers may devise experiments with multiple substances to track pathways of interaction during osmotic diffusion and polar directed migration through the medium that simulates pathways in a biological cell. The experiment with select substances may be conducted with controlled variations in medium, potential and temperature.

主权项

1. A method of performing moleculation that stimulates the conditions of an eukaryotic cell for potential molecular interactions comprising the steps of:
a. forming a stable, substrate medium adapted to accept a plurality of test samples having molecules with the characteristic of movement in the medium under the influence of an electric field, wherein the medium impedes movement of the molecules according to inherent characteristics of the molecules; b. establishing an electric field through the medium with an electropositive potential spatially displaced from an electronegative potential that is capable of inducing the vectorial molecular movement of the molecules in the test samples when placed in the medium between the electropositive potential and the electronegative potential, wherein the established electric field includes spaced, oppositely charged poles that are constructed to produce a non-orthogonal electro-potential field in the medium that induces non-parallel vectorial molecular movement of sample molecules in spatially separated test samples; c. placing two or more test samples in the medium at spatially separated locations, wherein the locations are selected relative to the established electric field between the electropositive potential and the electronegative potential such that the vectorial molecular movements of molecules in the respective spaced samples are such that the molecules migrate on paths in the medium under the influence of the electric field to move the separated molecules into closer proximity for potential molecular interactions.