| 主权项 |
1. A method of making an IRM conjugate comprising:
covalently attaching a first linker having a first functional group to an IRM compound having immunomodulatory activity, at a site on the IRM compound selected to preserve at least a portion of the immunomodulatory activity of the IRM compound, thereby forming an IRM moiety; and allowing the IRM moiety to react with a second moiety having biological activity and a second functional group, wherein a covalent bond is formed between the first functional group and the second functional group without use of UV irradiation, thereby forming an IRM conjugate that possesses IRM activity and the biological activity of the second moiety, and wherein the covalent bond formed by the reaction of the first functional group with the second functional group comprises a disulfide linkage, a thioether linkage, a hydrazone linkage, an oxime linkage, a urea linkage, a thiourea linkage, a hydrazine linkage, a hydroxyl amine linkage, or a nitrone linkage. |
