| 摘要 |
The present invention relates to methods for transferring gastrointestinal microbiota that preserves viability and bioactivity of the microbiota, even if fastidious, anaerobic, and non-culturable organisms are present. Also provided herein are examples of how manipulating the gastrointestinal microbiota and introducing particular taxa can be used to affect host metabolic status related to weight, fat, and obesity. |
| 主权项 |
1. A method for transfer of gastrointestinal microbiota from a donor subject to a recipient subject comprising the steps of:
(a) specimen collection, wherein a microbiota sample is recovered from the donor subject and, within 10 minutes of collection, is placed in an airtight collection container with or without an anaerobic transport medium, and sealed to avoid contact with oxygen in the air; (b) specimen preparation, wherein the microbiota sample collected in step (a) is prepared in an anaerobic environment, comprising (i) adding a reduced (no oxygen) sterile solution if the microbiota sample was not collected in solution in step (a) or optionally adding a reduced (no oxygen) sterile solution if the microbiota sample was collected in solution in step (a), followed by (ii) homogenization, (iii) removal of solids, and (iv) transfer to a transport container that is under an anaerobic environment and has an airtight cap; (c) transport of the microbiota sample prepared in step (b) to the delivery site in the recipient subject in the transport container; (d) removal of the microbiota from the transport container into a delivery vehicle with minimal oxygen exposure, and (e) direct transfer of the microbiota to the gastrointestinal tract of the recipient subject using the delivery vehicle, with minimal oxygen exposure. |
