| 摘要 |
Provided is a lymph cell amplification and activation method via serum-free cultivation. The method comprises: a) the lymph cell activation step, in which a biological sample containing lymph cells is put in contact with a lymph cell activation agent coated on a cultivation container and is cultivated for 3 to 6 days in a serum-free medium; b) a first amplification step, in which the culture obtained from step (a) is added with a serum-free medium having a volume 0.8 to 1.2 times of the culture, and is further cultivated for 1 to 3 days; c) a second amplification step, the culture obtained from step (b) is added with a serum-free medium having a volume 1 to 1.5 times of the culture, and is further cultivated for 1 to 3 days; d) a third amplification step, the culture obtained from step (c) is added with a serum-free medium having a volume 2 to 4 times of the culture, and is further cultivated for 4 to 6 days; e) a fourth amplification step, the culture obtained from step (d) is added with a serum-free medium having a volume 0.5 to 1.5 times of the culture, and is further cultivated for 4 to 6 days; f) the activated and amplified lymph cells from step (e) are obtained. |
