| 主权项 |
1. A microscopic method for generating a high-resolution image of a sample, wherein the method comprises the following steps:
a) providing said sample with a marker which emits statistically blinking luminescent radiation upon excitation, or a providing said sample having molecules that emit statistically blinking luminescent radiation upon excitation, b) exciting said the sample to luminescence by structured illumination such that said markers or said molecules emit luminescent radiation blinking at a blinking rate, wherein
the illumination is structured such that the blinking rate varies locally andthe sample is repeatedly illuminated in different illumination states of the structured illumination, such that a different local distribution of the blinking rate is obtained for each illumination state, c) repeatedly imaging said luminescing sample on a detector in each of the different illumination states, such that an image sequence is obtained for each of the different illumination states, d) generating a raw image reproducing the local distribution of the blinking rate in said sample from each image sequence, and e) generating a high-resolution image, which has a spatial resolution greater than that of the image on the detector from the obtained raw images by way of computational processing comprising Fourier filtering. |
