SYSTEMIC GENOTOXICITY AS BLOOD MARKER FOR ALLERGIC INFLAMMATION

摘要

The invention provides a method for detection of allergic inflammation in a subject that comprises assaying a test sample of peripheral blood from the subject for a marker of DNA damage. An elevated amount of marker present in the test sample compared to control sample is indicative of inflammation. The method can be adapted for quantitatively monitoring the efficacy of treatment of allergic inflammation in a subject. Markers of DNA damage include single- and/or double-stranded breaks in leukocytes, oxidative DNA damage in leukocytes, or a marker of nitric oxide oxidative activity (protein nitrosylation in leukocytes). This unexpected discovery of markers of systemic genotoxicity present in circulating leukocytes enables detection of allergic inflammation with a relatively simple and minimally invasive assay using peripheral blood.

主权项

1. A method for detection of inflammatory disease activity in a subject, wherein the inflammatory disease activity comprises allergic inflammation, the method comprising:
(a) contacting a test sample of peripheral leukocytes from the subject with reagents for assaying for a marker of DNA damage; (b) measuring the amount of marker present in the test sample as compared to a control sample; and (c) determining the presence of allergic inflammation when an elevated amount of marker is present in the test sample compared to the control sample.