siRNA Compositions and Methods for Inhibiting Gene Expression in Tumor Initiating Cells of breast Cancer

摘要

Disclosed are methods and compositions for siRNA-mediated therapy of mammalian diseases. In particular, compositions and methods are disclosed for treatment of therapy-resistant human breast cancers. In exemplary embodiments, siRNA molecules are presented that effectively knock down gene expression of one or more polynucleotides in breast cancer tumor initiating cells.

主权项

1. A small interfering RNA molecule that comprises:
(a) a duplex region; and (b) either no overhang region or at least one overhang region,
wherein each overhang region has six or fewer nucleotides,wherein the duplex region consists of a sense region and an antisense region that together form the duplex region, andwherein the antisense region and the sense region are each 19 to about 30 nucleotides in length, and the antisense region comprises a nucleotide sequence that is substantially complementary to the sequence of any one of SEQ ID NO: 1 to SEQ ID NO:32.