Methods of Modulating Smooth Muscle Cell Proliferation and Differentiation

摘要

The present disclosure provides methods of inducing smooth muscle cell differentiation. The present disclosure provides genetically modified cells comprising exogenous miR-143 and/or miR-145 nucleic acids; and artificial tissues comprising the genetically modified cells. The present disclosure provides methods and compositions for reducing pathological angiogenesis. The present disclosure provides methods of inducing therapeutic angiogenesis. The present disclosure provides methods, compositions, and devices for inhibiting vascular smooth muscle cell proliferation.

主权项

1. A method of inducing smooth muscle cell differentiation in a stem cell or progenitor cell, the method comprising introducing into a stem cell or a progenitor cell a micro-RNA-143 (miR-143) nucleic acid, a nucleic acid comprising a nucleotide sequence encoding a miR-143 nucleic acid, a micro-RNA-145 (miR-145) nucleic acid, a nucleic acid comprising a nucleotide sequence encoding a miR-145 nucleic acid, or one or more nucleic acids comprising nucleotide sequences encoding a miR-143 and a miR-145 nucleic acid, thereby generating a vascular smooth muscle cell.