METHODS AND COMPOSITIONS FOR MULTIPLEX PCR

摘要

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

主权项

1. A method of amplifying a plurality of different target nucleic acid molecules comprising:
contacting a plurality of different target nucleic acid molecules with a pool of target-specific primers having a cleavable group, and a polymerase under synthesizing conditions, and synthesizing a plurality of different target-specific molecules by extending one or more of the pool of target-specific primers having the cleavable group; digesting a plurality of the different target-specific molecules having the cleavable group of the target-specific primer; ligating at least one adapter to at least one of the plurality of digested different target-specific molecules; and producing at least one adapter-ligated amplified target sequence, wherein the method includes using no more than two target-specific primers to produce any one of the adapter-ligated amplified target sequences.