SPIDER SILK DRAGLINE POLYNUCLEOTIDES, POLYPEPTIDES AND METHODS OF USE THEREOF

摘要

The disclosure provides spider silk polypeptides and polynucleotides encoding the same. Methods of using such polypeptides and polynucleotides and designing novel biomaterials using repeat units of the polypeptides and polynucleotides.

主权项

1. A substantially purified polypeptide comprising an N-terminus sequence that is about 80-150 amino acids in length and having at least about 90% identity to SEQ ID NO:5 from amino acid position 71 to 151 followed by a sequence of SEQ ID NO:5 from position 1358-1384, wherein SEQ ID NO:5 from position 1358-1384 is preceded and/or followed by a glycine and alanine rich region and having a C-terminal region of about 50-100 amino acids in length and having at least about 90% identity to SEQ ID NO:5 from position 2085 to 2141, wherein the polypeptide has a property selected from the group consisting of (i) a Young's modulus of about 3.94 GPa, (ii) an Ultimate Strength of about 139 MPa, (iii) an Extensibility of about 0.818 mm/mm, (iv) a Toughness of about 66.7 MPa and (v) any combination of (i)-(iv).