发明名称 |
TRANSFORMED HUMAN PLURIPOTENT STEM CELLS AND ASSOCIATED METHODS |
摘要 |
The present disclosure provides transformed human pluripotent stem cell (t-hPSC). t-hPSCs are not dependent on Oct4 for renewal and survival, however exhibit a sensitivity to reduced levels of the transcription factor Nanog. Also provided are methods of culturing cells for use in a cell-based screening assay comprising placing one or more transformed human pluripotent stem cells into a receptacle and culturing said stem cells in the receptacle to form a monolayer of stem cells without cell overlap. Methods of screening compounds using t-hPSCs are also described. |
申请公布号 |
US2015038371(A1) |
申请公布日期 |
2015.02.05 |
申请号 |
US201414329412 |
申请日期 |
2014.07.11 |
申请人 |
McMaster University |
发明人 |
Bhatia Mickie;Werbowetski-Ogilvie Tamra;Sachlos Eleftherios;Fischer Russell Daniela;Laronde Sarah;Lee JungBok;Szabo Eva;Risueno Ruth |
分类号 |
C12N5/0735;C12Q1/68;C12N5/095;G01N33/50;G01N33/569 |
主分类号 |
C12N5/0735 |
代理机构 |
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代理人 |
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主权项 |
1. A transformed human pluripotent stem cell (t-hPSC). |
地址 |
Hamilton CA |