High-Throughput Method For Sialic Acid Quantitation

摘要

The present invention relates to a method for specifically measuring sialylation of a biomolecule of interest without the interference of other biomolecules present in the sample.

主权项

1. A method of detecting sialylation of a biomolecule of interest in a sample comprising:
(a) purifying the biomolecule of interest by contacting the sample with an agent that binds the biomolecule of interest; (b) denaturing the biomolecule of interest by incubating the sample with a surfactant; (c) contacting the denatured sample with an agent capable of removing terminal sialic acid residues from the biomolecule of interest, thereby generating free sialic acid residues; (d) labeling the free sialic acid residues with a detectable label; (e) detecting the labeled sialic acid residues, thereby detecting sialylation of the biomolecule of interest; whereby said method allows for the detection of sialylation of the biomolecule of interest without interference from host cell proteins or impurities.