Modulation of hsp47 expression

摘要

Compositions, methods and kits for modulating expression of target genes are provided herein. In various aspects and embodiments, compositions, methods and kits provided herein modulate expression of heat shock protein 47 (hsp47 or SERPINH1). Said compositions, kits or methods may involve use of short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA) or short hairpin RNA (shRNA)) that bind hsp47. Also provided are compositions, methods and kits for treating and/or preventing diseases, conditions or disorders associated with hsp47. WO 2011/072082 PCT/US2010/059578 1.4 H 293_gfp 12 293_hsp47gfp#2 E 293_gp46gfp #1 5 1.0 HT1080_gfp HT1080_hsp47gfp M HT1080_gp46gfp .0.6 E hTERTgfp hTERT hsp47gfp 0 m NRKgfp S0.2 NRKgp46gfp Treatment: 1. cells only 2. RNAiMAX 3. RNAiMAX +- scrambled siRNA 4. RNAiMAX +v siGFP