MONITORING IMMUNE RESPONSIVENESS TO CANCER VACCINATION

摘要

The invention is direct to a method for determining a cancer patient's immune responsiveness to anti-cancer vaccination. In one aspect, for each of a plurality of vaccinations, pairs of clonotype profiles are obtained, one immediately prior to vaccination and one during the period of peak immune response, usually within two to twenty days after the vaccination. Responsiveness is correlated to successive increases in identical clonotypes within each pair of clonotype profiles in at least two successive vaccinations.

主权项

1. A method of measuring immune responsiveness of a patient to a cancer vaccine, the method comprising the steps of:
generating a pair of clonotype profiles at each of a plurality of successive vaccinations of a patient with a cancer vaccine, wherein a first clonotype profile of each pair is from a sample from the patient prior to vaccination and a second clonotype profile of each pair is from a sample from the patient after vaccination at a time within a peak immune response to vaccination, each clonotype profile comprising at least 1000 sequence reads of at least 30 nucleotides; and correlating immune responsiveness of the patient to the cancer vaccine with an increase of identical clonotypes within each pair of clonotype profiles of at least two successive vaccinations.