ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS

摘要

A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

主权项

1. A method for producing a transgenic plant or plant tissue cell, the method comprising:
providing a nucleic acid molecule comprising at least two polynucleotides selected from the group consisting of SEQ ID NOs:11-14, 17, and 18, and at least one targeting endonuclease recognition site, wherein the at least two-polynucleotides selected from the group consisting of SEQ ID NOs: 11-14, 17, and 18 flank the at least one targeting endonuclease recognition site; and transforming the plant cell—with the nucleic acid molecule, wherein the nucleic acid molecule is stably integrated into the genome of the plant cell; and generating plant tissue or regenerating a plant from the plant cell transformed with the nucleic acid molecule.