MODIFIED BACTERIAL CELL

摘要

The disclosure relates to a Gram negative bacterial cell that is transformed with a nucleic acid molecule that encodes a Gram positive twin-arginine translocase and including methods for the production of polypeptides.

主权项

1. A Gram-negative bacterial cell wherein said cell is genetically modified by transformation with a nucleic acid molecule encoding a polypeptide complex comprising a twin arginine translocase [Tat] system isolated or made from a Gram positive bacterial species and further wherein said Gram negative bacterial cell expresses a polypeptide which polypeptide is adapted to interact with said Tat complex and is secreted into the cell culture medium.