FLUORESCENT PROTEIN-BASED INDICATORS

摘要

The presently-disclosed subject matter includes fluorescent protein-based indicators for detecting ions, small molecule analytes, or combinations thereof. In some embodiments the indicators include a polypeptide, which itself includes a fluorescent polypeptide, a compound-binding polypeptide, and a polypeptide target of the compound-binding polypeptide. In some embodiments the polypeptide includes an EosFP polypeptide, a calmodulin polypeptide, and a M13 polypeptide, or fragments and/or variants thereof. The presently-disclosed subject matter also includes methods for detecting calcium in a sample with embodiments of the present polypeptides. In some embodiments the present indicators experience a permanent shift from green to red fluorescent when exposed to an detecting substance, such as calcium.

主权项

1. An isolated polypeptide, comprising:
an EosFP polypeptide, which includes an C-terminus portion comprising the sequence of SEQ ID NO. 2 and a N-terminus portion comprising the sequence of SEQ ID NO. 3, or a variant and/or fragment thereof; a calmodulin (CaM) polypeptide, comprising the sequence of SEQ ID NO. 4, or a variant and/or fragment thereof; and a M13 polypeptide, comprising the sequence of SEQ ID NO. 5, or a variant and/or fragment thereof.