发明名称 |
METHOD FOR DETECTING DEGREE OF MALIGNANCY OF CIRCULATING TUMOR CELL UNIT, AND KIT FOR SAME |
摘要 |
The object of the present invention is to provide an evaluation method capable of accurately determining a metastasis of cancer, the stage of cancer progression, or the malignancy of cancer.
The object can be solved by a method for detecting the degree of malignancy of each of the circulating tumor cells, characterized by the following steps:(a) bringing an epithelial cell-binding component, which specifically binds to a marker molecule expressed on epithelial cells and is fluorescently-labeled or luminescent enzyme-labeled, and a mesenchymal cell-binding component, which specifically binds to a marker molecule expressed on mesenchymal cells and is fluorescently-labeled or luminescent enzyme-labeled, into contact with a sample that possibly contains circulating tumor cells,(b) detecting a fluorescence signal or a luminescence signal of the epithelial cell-binding component and a fluorescence signal or a luminescence signal of the mesenchymal cell-binding component of each of the cells, and (c) determining the degree of epithelial-mesenchymal transition of circulating tumor cells based on the signal amount of the epithelial cell-binding component (E) and the signal amount of the mesenchymal cell-binding component (M). |
申请公布号 |
EP2833149(A1) |
申请公布日期 |
2015.02.04 |
申请号 |
EP20130770163 |
申请日期 |
2013.03.28 |
申请人 |
ON-CHIP BIOTECHNOLOGIES CO., LTD. |
发明人 |
TAKEDA, KAZUO;YAMASHITA, NAMIKO;FUJIMURA, YUU;NISHIO, KAORI;KOH, YASUHIRO;WATANABE, MASARU;KOIZUMI, FUMIAKI;UEHARA, YURI |
分类号 |
G01N33/574;C07K14/47;C12N5/07;G01N33/536;G01N33/543 |
主分类号 |
G01N33/574 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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