| 发明名称 | Whole cell biocatalyst | ||
| 摘要 | The present invention relates to a method for producing a product of a reaction catalysed by a nitrilase, which method comprises the steps (i) providing a microorganism comprising said nitrilase located on its surface, and/or a membrane preparation of said microorganism, and (ii) contacting the microorganism and/or the membrane preparation thereof with one or more nitrilase substrates under conditions compatible with nitrilase activity. The present invention further relates to a method for producing enantiomerically pure (R)-mandelic acid using the nitrilase-displaying whole cell biocatalyst or membrane preparation thereof for the conversion of racemic mandelonitrile. | ||
| 申请公布号 | US8945887(B2) | 申请公布日期 | 2015.02.03 |
| 申请号 | US201013509935 | 申请日期 | 2010.11.16 |
| 申请人 | Zyrus Beteiligungsgesellschaft mbH & Co. Patente I KG | 发明人 | Jose Joachim;Maas Ruth;Detzel Christian |
| 分类号 | C12N15/70;C12P7/42;C12N9/78 | 主分类号 | C12N15/70 |
| 代理机构 | Greenberg Traurig, LLP | 代理人 | Greenberg Traurig, LLP |
| 主权项 | 1. A method comprising the steps of: (i) providing a microorganism comprising a recombinant nitrilase located on its surface, and/or on a membrane preparation of said microorganism, (ii) contacting the microorganism and/or the membrane preparation thereof with one or more nitrilase substrates under conditions compatible with nitrilase activity, wherein the nitrilase is fused to a transporter domain of an autotransporter. | ||
| 地址 | DE | ||